This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the manuscript and its Supporting Information files.įunding: o This work was funded by the Science and Technology Research Partnership for Sustainable Development (SATREPS), the Japan International Cooperation Agency (JICA), and the Japan Agency for Medical Research and Development (AMED) (Grant No. Received: JAccepted: OctoPublished: November 4, 2020Ĭopyright: © 2020 Yoshikawa et al. PLoS Negl Trop Dis 14(11):Įditor: Gregory Gromowski, WRAIR, UNITED STATES
Although the Direct RT-LAMP assay was less sensitive than the standard RT-LAMP, the Direct RT-LAMP assay can be available as the rapid first screening of COVID-19 in poorly equipped areas, such as rural areas in developing countries.Ĭitation: Yoshikawa R, Abe H, Igasaki Y, Negishi S, Goto H, Yasuda J (2020) Development and evaluation of a rapid and simple diagnostic assay for COVID-19 based on loop-mediated isothermal amplification. We also developed the RT-LAMP assay without the RNA extraction step–Direct RT-LAMP, which could detect more than 1.43 x 10 3 copies within 15 min with a 100% probability in clinical evaluation test.
BIOEDIT GLASSDOOR PORTABLE
Since the RT-LAMP assay can be performed with a portable battery-supported device, it is a rapid, simple, and sensitive diagnostic assay for COVID-19 that can be available at point-of-care. Clinical evaluation of nasal swab samples from suspected SARS-CoV-2 pneumonia (COVID-19) patients showed that the assay could detect over 23.7 copies within 15 min with a 100% probability. This RT-LAMP assay specifically detected SARS-CoV-2 without cross-reacting with the most closely related human coronavirus, SARS-CoV. Here we describe a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that uses a portable device for efficient detection of SARS-CoV-2. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly pathogenic novel coronavirus that has caused a worldwide outbreak.